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Dynamics of Ste2p(G31-T110; TM1-TM2) in TFE:water. A) 15N{1H}-NOE of [15N]-TM1-TM2 in TFE:water at 25°C. Values above 0.6 (bottom line) and above 0.75 are indicative of rigidity or increased rigidity, respectively. B) H-D exchange analysis of [15N]-TM1-TM2 in TFE-d3:D2O+0.1%TFA-d. The gray boxes indicates locations of the predicted helices from the LPPG structure [1].
