Table 1. Real-time RT-PCR results for selected genes.
| Microarray | Real-time RT-PCR | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene name | Function | FC | |||||||
| CH | Combo | DOX | CBZ | CH | Combo | DOX | CBZ | ||
| DNA-damage-inducible transcript 4 (Ddit4) | Hypoxic stress response; cell growth | 7.4 | −6.2 | −5.6 | - | 13.9 | −2.5 | −5.7 | - |
| Matrix metalloproteinase 3 (Mmp3) | Extracellular matrix remodeling | 7.4 | −3.6 | −2.9 | - | 5.3 | −1.5 | −3.5 | −2.5 |
| Metallothionein 1 (MT2) | NO-mediated signal transduction | 15.6 | −6.8 | −5.5 | - | 19.7 | −2.8 | −14.9 | - |
| Tubulin, alpha 4 (Tuba4) | Microtubule-based movement | −6.2 | 5.8 | 5.8 | - | −26.0 | 27.9 | 36.8 | - |
| GATA binding protein 4 (Gata4) | Transcription regulation; heart development | −3.0 | 4.0 | 2.7 | 2.1 | −3.3 | 8.6 | 4.9 | 2.0 |
| Serine protease inhibitor 2-2(Spi2-2) (Serpin3n) | Acute-phase response; inflammation | 40.5 | −12.7 | −9.7 | −2.3 | 90.5 | −18.4 | −3.8 | −13.9 |
| Transformation related protein 53 inducible nuclear protein 1 (Trp53inp1) | Stress response; apoptosis | 4.8 | −5.0 | −3.5 | - | 6.1 | Red | −1.9 | −3.0 |
| NADPH oxidase 4 (Nox4) | Electron transport; superoxide release | 4.3 | −3.4 | −3.2 | −1.9 | 22.6 | −2.5 | −4.0 | −3.5 |
| Gem GTPase (Gem) | Calcium channel blockage | 2.7 | −2.9 | −2.8 | - | 8.0 | −1.7 | −1.8 | - |
| Oncostatin receptor (Osmr) | Inflammation; connective tissue production; extracellular matrix turnover | 5.1 | −2.9 | −2.6 | −1.7 | Ind | Red | Red | Red |
| Phospholipase A2 group VII (platelet-activating factor acetylhydrolase, plasma) (Pla2g7) | Inflammation; lipid catabolism | 3.7 | −2.3 | −2.2 | - | 7.5 | −2.0 | −1.9 | −3.5 |
| SET and MYND domain containing 1 (Smyd1) | Heart development | −2.9 | 3.2 | 2.7 | 1.7 | −1.7 | 4.9 | 1.6 | 3.3 |
| Lipocalin 2 (Lcn2) | Vascular remodeling; apoptosis | 27.7 | −16.6 | −13.3 | −1.6 | 64.0 | −7.0 | −9.9 | - |
| Cyclin-dependent kinase inhibitor 1A (p21) (Cdkn1a) | Cell cycle arrest | 14.6 | −8.6 | −6.5 | - | 128.0 | −13.0 | −7.0 | −2.5 |
| S100 calcium binding protein A8 (calgranulin A) (S100a8) | Cell proliferation; calcium signaling | 7.1 | −14.3 | −19.9 | - | 5.7 | −137.2 | −181.0 | −2.1 |
| S100 calcium binding protein A9 (calgranulin B) (S100a9) | Cell proliferation; calcium signaling | 7.1 | −15.9 | −14.0 | - | 17.2 | −52.0 | −104.0 | - |
| Cyclin G2 (Ccng2) | Cell cycle regulation | 3.4 | −3.8 | −3.1 | - | 9.2 | −2.6 | −5.7 | - |
| Cytokine inducible SH2-containing protein 3 (Socs3) | Regulation of cell growth; negative regulation of insulin signaling | 8.2 | −7.9 | −4.9 | −2.4 | 9.9 | −4.0 | −4.0 | −4.3 |
N: normal mice; CH: ISO-treated mice; DOX: mice treated with ISO + DOX; CBZ: mice treated with ISO + CBZ, and Combo: mice treated with ISO + DOX + CBZ. FC: fold change. Ind/Red (Induced/reduced) are used instead of fold changes where no transcript was detected in one of the two samples being compared.