Table 1.
Donor MHC class II+ CFU-C in the bone marrow of BN recipients after liver, heart, or bone marrow transplantation
Graft/cells | Days after transplantation | n | Donor MHC class II+ CFU-C counts (per 5×105 BMC) | Mean |
---|---|---|---|---|
Bone marrow | 14 | 3 | 4.1, 5.3, 6.8 | 5.4 |
30 | 2 | 21.5, 30.2 | 25.9 | |
60 | 2 | 6.3, 9.3 | 7.8 | |
100 | 4 | 0, 0, 0, 0 | 0 | |
Liver | 14 | 4 | 0.3, 0.6, 1.5, 1.5 | 1.0 |
30 | 2 | 0.1, 0.5 | 0.3 | |
60 | 2 | 0,0 | 0 | |
100 | 2 | 0,0 | 0 | |
Heart | 14 | 2 | 0,0 | 0 |
Plastic dish-nonadherent BMC from BN recipients after LEW bone marrow (2.5×108), liver, or heart transplantation were cultured in the fibrin clot culture system (5×105 cells/plate, 10 plates/each experiment). After culturing for 6 days, established CFU-C were in situ stained with mAb L21–6 (LEW MHC class II), and the number of CFU-C composed of strongly stained cells was counted.