Figure 4.

Altered gene expression and iodide uptake in PBF-Tg thyroids. A, Serum TSH analyses in 6-week old PBF-Tg and WT mice; N = 6. Relative mRNA expression of indicated growth factor and TSHR in either PBF-Tg thyroids (B) or primary thyrocyte cultures (C) compared to WT; N = 6-15. D, Detection of TSHR isoforms α and β by Western blot analyses in PBF-Tg and WT thyroids; N = 4. E, Relative mRNA expression of PTTG, TG, TTF1, PAX8 and NIS in PBF-Tg thyroids compared to WT; N = 6-13. F, Detection of NIS by immunohistochemistry of PBF-Tg and WT thyroids. Scale bars: 100 μm. G, Relative ¹²⁵I uptake in primary thyrocyte cultures from PBF-Tg and WT mice; N = 5-6. Detection of PBF-HA expression by Western blot analyses (H) and relative ¹²⁵I uptake (I) in primary PBF-Tg thyrocyte cultures either non-transfected (UT) or transfected with PBF and scrambled (Scr) siRNA as indicated; N = 8-20. Data presented as mean ± SE