Figure 6.

Functional interaction between PBF and NIS in human thyrocytes. A, Quantification of PBF mRNA expression in MNG (N = 24) relative to normal thyroid (N = 11). Data presented as a scatterplot. B, Detection of PBF and TSHR expression by Western blot analyses in MNG and normal thyroids. C, Quantification of NIS mRNA expression in MNG (N = 24) relative to normal thyroid (N = 11). Data presented as a scatterplot. D, Correlation of PBF and NIS mRNA expression (shown as ΔCT values) in MNG and normal thyroid. Statistics analysed using Spearman rank correlation. E-G, Detection of PBF expression by Western blot analyses (E), quantification of NIS mRNA expression (F) and relative ¹²⁵I uptake (G) in primary human thyrocyte cultures transfected with either PBF or Scr siRNA as indicated; N = 9-11. Data presented as mean ± SE.