Figure 4. Effects of selective PI3K p110β inhibition.

Ex vivo human lung fibroblasts (from three different donors) were cultured, and treated with TGX-221 following the protocol indicated in Figure 1. A) Representative western blot analysis of phosphorylated (Ser⁴⁷³) Akt and pertinent graph with pAKT/α-tubulin ratios of three separate experiments. B) Results of cell counting by optical microscopy (grey columns) and WST-1 cell proliferation assay (black columns) were represented as % increase over control. C) Flow-cytometry analysis of α-SMA expression: MFI mean values (± sd) of three separate experiments are indicated. Plots of one representative experiment are shown in FigureS3. D) Total collagen levels, were reported as % increase over control. Statistical significance across treatment groups was determined using the one-way ANOVA. A P value <0.05, which indicates a statistically significant difference among relevant groups, is designated with an asterisk.