Figure 2. Expression of the GR transgene caused apoptosis.

(A) DMS 79 cells were infected with either GR-eYFP or eYFP retroviruses and GR protein assessed at 72 h. (B) DM79 cells treated as above were fixed for immunohistochemistry to detect cleaved caspase-3. Cells were stained for activated caspase-3 and then analysed for co-localisation with either GR-eYFP or eYFP. Percentage of cells positive for cleaved caspase-3 and eYFP or GR-eYFP. Cell counts derived from 3 independent infections and represented as percentage of caspase-3 positive to eYFP positive as mean +/− S.E.M. (n = 3) with *** indicating p<0.001, Student's t-test for independent samples.