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. 2011 Oct 3;6(10):e25336. doi: 10.1371/journal.pone.0025336

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Mitchell et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) eaf1Δ mutants exhibit elongated buds that can be suppressed by deletion of the Swe1 morphogenesis checkpoint. Wild type (YKB779), eaf1Δ (YKB42), swe1Δ (YKB1807), and eaf1Δ swe1Δ (YKB1266) cells were grown to mid-log phase in YPD at 25°C, fixed, and bud morphology was scored for at least 100 cells of each type. The average of three replicates is presented. (B) Deletion of SWE1 rescues the temperature sensitivity of eaf1Δ cells. The strains used in (A) were spotted in ten-fold serial dilutions and incubated at the temperatures indicated on YPD plates. (C) Overexpression of SWE1 is toxic to eaf1Δ cells. Wild type (YKB779), eaf1Δ (YKB42), pGAL-SWE1 (YKB1648), and eaf1Δ pGAL-SWE1 (YKB1806) cells were spotted on complete medium (yeast extract, peptone) in 10-fold serial dilutions containing glucose or galactose as the sugar source at 25°C for 2 days.