Table 1. Quantification of defects in eaf1Δ cells.

Morphology		WT	eaf1Δ
Bud	Septin	(n = 15)	(n = 43)
Normal	Normal	100%	65%
Elongated	Mis-localized in bud	0%	19%
No bud	Ring did not re-assemble (>1 hour)	0%	11%
Normal	Localized to one side of neck	0%	5%

Using time lapse imaging of cells expressing Cdc11-GFP in the presence (WT (YKB1312)) or absence of EAF1 (eaf1Δ (YKB1310)), mother cells (immediately after cytokinesis) were followed through a cell cycle and bud and septin morphology was scored. Images were collected at five or fifteen minute intervals for wild type and eaf1Δ cells, respectively, at room temperature.