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. 2011 Oct 3;6(10):e25280. doi: 10.1371/journal.pone.0025280

Figure 3. Sec13 and Sec28 co-precipitate with calcineurin A from cell lysates.

Figure 3

A. A co-IP of the GFP-Sec13 with the mCherry-Cna1 using the RFP-Trap resin. A strain that expresses only GFP-Sec13 and a strain that expresses only mCherry-Cna1 served as negative controls. The membrane was initially probed with an anti-GFP antibody, stripped and subsequently probed with an anti-dsRed antibody to detect the precipitated mCherry-Cna1. B. A co-IP of GFP-Sec28 with mCherry-Cna1 using the RFP-Trap resin. A strain that expresses only GFP-Sec28 and a strain that expresses mCherry-Cna1 but not GFP-Sec28 served as negative controls. The membrane was probed with an anti-GFP antibody and subsequently probed with an anti-dsRed antibody to detect the precipitated mCherry-Cna1.