Table 2. Strains and plasmids used in this study.
| Strain or plasmid | Relevant propertiesa | Source or reference |
| S. flexneri | ||
| 2457T | wild-type strain | ISPb |
| MSF107 | 2457T Δwzz pHS2::aph, KmR | [50] |
| MSF102 | 2457T ΔwzzB | [50] |
| MSF209 | 2457T ΔwzzB Δwzz pHS2::aph, KmR | [50] |
| MSF1749 | 2457T Δwzy | This study |
| MSF1210 | 2457T ΔwaaL | This study |
| MSF1144 | 2457T ΔwaaD | This study |
| MSF1009 | 2457T ΔwaaI | This study |
| MSF1015 | 2457T ΔwaaJ | This study |
| MSF2743 | 2457T ΔgtrABII | This study |
| MSF1650 | 2457T ΔarnT | This study |
| MSF1666 | 2457T ΔpmrA | This study |
| Plasmids | ||
| pKD46 | bla PBAD gam bet exo pSC101 oriTS | [57] |
| pKD4 | bla FRT aph FRT PS1 PS2 oriR6K | [57] |
| pCP20 | bla cat cI857 λPR flp pSC101 oriTS | [58] |
| pGEM-T-Easy | TA cloning vector, ApR | Promega |
| pJC139 | S. flexneri 2457T wzzB cloned into pGEM-T-Easy ApR | [63] |
| pJC142 | S. Typhimurium LT2 wzz fepE cloned into pGEM-T-Easy ApR | [67] |
| pMM110 | S. Typhimurium LT2wzz LT2 cloned into pGEM-T-Easy, ApR | This study |
| pJC144 | S. flexneri 2457T wzz pHS2 cloned into pGEM-T-Easy, ApR | [66] |
| pMM112 | S. flexneri 2457T waaL cloned into pGEM-T-Easy, ApR | This study |
| pMM113 | S. flexneri 2457T eptA cloned into pGEM-T-Easy, ApR | This study |
| pCC1 | Single copy cloning vector, oriII, oriV, CmR | EPICENTRE |
| pMM111 | S. flexneri 2457T gtrABII cloned into pCC1, CmR | This study |
| pRMCD108 | WzzB(K267N) | [68] |
| pRMCD127 | WzzB(M32T) | [68] |
a
KmR, kanamycin resistant; ApR ampicillin resistance; CmR, chloramphenicol resistance.
b
ISP, Institute of Public Health, Santiago, Chile.