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. 2011 Oct 3;6(10):e25590. doi: 10.1371/journal.pone.0025590

Figure 4. Gene-level analysis of intra-twin discordance in DNA methylation.

Figure 4

The discordance in DNA methylation levels was calculated within monozygotic twin pairs, dizygotic twin pairs and between non-related individuals (MZ, DZ and NR, respectively). The methylation differences between individuals were summed over a region and displayed as scatter plots with a horizontal black line representing the group median (see Figures S2, S3, S4, S5, S6 for intra-twin comparisons at each individual CpG unit). Significant differences between groups were identified using the non-parametric Kruskal-Wallis test, followed by a Dunn's multiple comparison test. In H19-ICR, the methylation discordance was significantly less in MZ twin pairs than in DZ pairs, or between non-related individuals. Within the RUNX1 promoter, the non-related individuals displayed the greatest discordance over MZ or DZ twin pairs (*: P-value<0.05; **: P-value<0.01; ***: P-value<0.001).