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. 2011 Oct 3;6(10):e25369. doi: 10.1371/journal.pone.0025369

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Willis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Left panel: Liposome co-pelleting assays using multilamellar vesicles (DOPS/DOPE at molar ratio of 3∶1) at neutral pH shows binding of both SAPLIPs independent of metal ions. While for Na-SLP-1 full binding is observed, Ac-SLP-1 binding levels are ∼20%. Middle/right panels: Adsorption of both SAPLIPs to monolayers (DOPS/DOPE at molar ratio of 1∶3) was assessed in a Langmuir surface film balance at neutral (pH 7.5, blue curves) and acidic conditions (pH 4.7, red curves). While quantitatively different, three phases are observed for both proteins under all tested conditions: an immediate-onset Langmuir-like adsorption with small surface-pressure increase (phase 1, peripheral binding), followed by a large surface pressure increase (phase 2, embedding into the membrane), and a final stage with either stable (Ac-SLP-1) or decreasing (Na-SLP-1) surface pressure (phase 3).