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. 2011 Oct 3;6(10):e25510. doi: 10.1371/journal.pone.0025510

Figure 4. Sequences of sense single-strand DNA obtained with R2CNA.

Figure 4

A) Schematic representation of the strategy used for cloning. The sense single strand DNA were purified and ligated with RNA ligase. A second PCR was performed with forward (red) and reverse (blue) primers surrounding the position of ligation. These PCR products were then cloned and sequenced. B) Sequences obtained for each polymerase with the R2CNA primer. The nucleotides corresponding to the terminal transferase activity of Taq DNA polymerase are indicated in blue and incorporation of nucleotides is shown in red. The length of these fragments is indicated.