Table 1. Sequences of the primers used in PCR.
Primer | Sequence |
F | 5′-ggtattgagggtcgcatc-3′ |
R | 5′-agaggagagttagagccatc-3′ |
RW | 5′-acatcgcatacttatact T agaggagagttagagccatc-3′ |
RCNA | 5′-acatctcatacttatact T*T agaggagagttagagccatc-3′ |
R2CNA | 5′-atctcatacttatact T*TT*T agaggagagttagagccatc-3′ |
R2Peg | 5′-atctcatacttatact PegPeg agaggagagttagagccatc-3′ |
α,β-D-CNA TT is indicated by T*T and triethylene glycol phosphate unit by Peg in the primer sequences. The modification of lengths due to the insertion of α,β-D-CNA TT or triethylene glycol phosphate unit in primers RCNA and R2CNA, correspond to the addition of 2, and 4 nucleotides equivalent, respectively.