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. 2011 Oct 3;6(10):e25510. doi: 10.1371/journal.pone.0025510

Table 1. Sequences of the primers used in PCR.

Primer Sequence
F 5′-ggtattgagggtcgcatc-3′
R 5′-agaggagagttagagccatc-3′
RW 5′-acatcgcatacttatact T agaggagagttagagccatc-3′
RCNA 5′-acatctcatacttatact T*T agaggagagttagagccatc-3′
R2CNA 5′-atctcatacttatact T*TT*T agaggagagttagagccatc-3′
R2Peg 5′-atctcatacttatact PegPeg agaggagagttagagccatc-3′

α,β-D-CNA TT is indicated by T*T and triethylene glycol phosphate unit by Peg in the primer sequences. The modification of lengths due to the insertion of α,β-D-CNA TT or triethylene glycol phosphate unit in primers RCNA and R2CNA, correspond to the addition of 2, and 4 nucleotides equivalent, respectively.