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. 2011 Oct 3;6(10):e25655. doi: 10.1371/journal.pone.0025655

Figure 1. Effects of IRS-1 constitutive expression on myogenic differentiation in L6 myoblasts.

Figure 1

A: Differentiation of L6 myoblasts stably expressing GFP (L6-GFP) or myc-IRS1 (L6-mIRS1) was induced by changing medium from 10% FBS-DMEM to 2% FBS-DMEM. At 0 or 6 days after induction of differentiation, cell morphology was shown. B: Differentiation of L6-GFP or L6-mIRS1 cells was induced. Cells were fixed on 6 days after induction of differentiation and stained with DAPI (blue) or phalloidine (red). C: Differentiation of L6-GFP cells or L6-mIRS1 cells was induced. Cells were lysed on the indicated day (0, 1, 2, 3, 4 or 6: days after induction of differentiation). Ten µg of total cell lysates was separated by SDS-PAGE, and subjected to immunoblotting analyses with indicated antibodies (IB). These are representative immunoblots independently performed three times.