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. 2011 Oct 3;6(10):e25655. doi: 10.1371/journal.pone.0025655

Figure 5. Effects of Foxo1 (Δ256) expression on myogenic differentiation.

Figure 5

A: Differentiation of L6 myoblast cells were induced by changing medium from 10% FBS-DMEM to 2% FBS-DMEM. During induction of differentiation, various concentrations of SB216763 (a specific inhibitor to GSK3) or LiCl were added to the medium. Cells were harvested at the indicated day after induction of differentiation. Immunoblotting analyses were carried out using indicated antibodies (IB). These are representative immunoblots independently performed three times. B: Differentiation of L6 myoblast cells stably expressing dominant interfering form of Foxo1 (L6-Δ256Foxo1) and cells infected with mock retrovirus vector (L6-mock) were induced by exchanging medium containing 2% FBS from 10% FBS. Cells were lysed on the indicated day (0, 1, 2, 4 or 6: days after induction of differentiation). Ten µg of total cell lysates were separated by SDS-PAGE, and subjected to immunoblotting analyses with indicated antibodies (IB). These are representative immunoblots independently performed three times. C: At 6 days after induction of differentiation, cell morphology was shown.