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. 2011 Oct 3;6(10):e25655. doi: 10.1371/journal.pone.0025655

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Hakuno et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: L6-mock or L6-mIRS1 was incubated in DMEM containing 2% FBS for 18 h and 6 days. Cells were immunostained with anti-Foxo1 antibody. Foxo1 localization is shown. B: Satellite cells were separated from the rat soleus muscle and incubated in DMEM containing 20% FBS. Plasmid expressing myc-tagged Foxo1 H215R mutant was transfected into satellite cells. One day after transfection, muscle differentiation was induced by changing medium to 2% FBS. One day after induction of differentiation, cells were fixed, permeabilized and immunostained with anti-myc antibody. DAPI staining was shown in blue. Myc staining (Foxo1) is shown in green.