Table 1. Primer sequences and conditions for amplification of VSX2 exons.

Set/exon	Forward primer sequence	Reverse primer sequence	PCR product	Annealing temp/ special conditions
Set 1/exon 1	TCCAGAGCATTAGACACCGG	TGGCAGGAACTTTTCCGCCT	603 bp	55 °C (5% DMSO; 20% Betaine)
Set 2/exon 2	GTTCAAAACCTCCGGATTCG	TCCGTTGTCGGCGAAAATAG	392 bp	55 °C
Set 3/exon 3	TCTTGTCTGAGACAGGCTCT	TCATGGGCATCTGGAACCCT	268 bp	55 °C (5% DMSO; 20% Betaine)
Set 4/exon 4	CACCATGGAGTAGGCGAGCT	ATTTCTCTCCTGCTAGGCTG	432 bp	55 °C
Set 5/exon 5	CAGTTCAAGATGGCTTTCCC	ATGTCTCAGCATGGTCCAGA	574 bp	55 °C