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. 2011 Oct 3;6(10):e25802. doi: 10.1371/journal.pone.0025802

Figure 1. Stable and transient transformation of P. japonicum mediated by A. rhizogenes.

Figure 1

Transformation induced by A. rhizogenes ATCC15834. A. Five-week-old plants showing accumulation of black substance(s) at the wound site after hypocotyl-cutting infection method. B. Transformed roots emerged from cotyledons 5 weeks after A. rhizogenes inoculation by the SAAT method. The black arrow indicates transformed roots. C and D GFP-fluorescing transformed roots observed under bright field (C) or fluorescent (D) microscopy. The black arrows point to fluorescent roots and the white arrow to non-fluorescent root. E and F Transiently-transformed cotyledons observed under bright field (E) or fluorescent (F) microscopy. G and H Confocal micrograph of cotyledon leaves. Non-transformed (G) and transiently-transformed (H). Red color corresponds to autofluorescence from chlorophyll. White bars correspond to 2 mm and yellow bars to 20 µm.