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. 2011 Oct 3;6(10):e25802. doi: 10.1371/journal.pone.0025802

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Ishida et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Frequency of stable transformation in P. japonicum seedlings submitted to different treatments. A Transformation efficiency in 3-day-old seedlings infected with strains ATCC15834, LBA1334 and AR1193 and co-cultivated for 2 or 7 days. The data show representative results from one of two independent experiments using 20 to 60 plants each. B Effect of acetosyringone (AS) on transformation efficiency. Seedlings were co-cultivated with A. rhizogenes strains ATCC15834 and LBA1334 in media with or without 100 µM AS for 2 days for LBA1334, and 7 days for ATCC15834. 4–5 weeks after the inoculation the transformation frequency was scored. The data show representative results from one of at least two independent experiments using 20 to 60 plants each.