Figure 2. GSH supplementation alters inflammatory cytokine release.

The J774 (A) and primary AM (B) were grown in media with or without 500 µM GSH and treated with LPS for 2 hours. TNFα release was analyzed in the media by ELISA. J774 nuclear fractions were examined for the nuclear translocation of the p65 subunit of NF-κB (C), HDAC used as a protein loading control with the ratio of p65 to HDAC area density listed below. Data represented as mean ± SEM, with ***p<0.001 compared between PBS and GSH.