Figure 2. Bioenergetics of wildtype and ATP6¹ animals.

A) P-Arg:Arg ratios exhibit impaired bioenergtics in ATP6¹ animals only at adult day 3 and 6 compared to age-matched wildtype. All mutant genotypes are mt ATP6¹, sesB¹/+ and wildtype controls are mt ATP6⁺, sesB/+. sesB¹ (recessive stress sensitive B mutation) is the fly homologue to ANT (adenine nucleotide translocase) and ATP6¹ is maintained in this mutant background and the heteroplasmy is verified by RFLP analysis prior to experimentation (Data not shown). F1 female progeny heterozygous for sesB¹ are of high mutant heteroplasmy and were analyzed compared to sesB¹ heterozygote controls. N = 9 wildtype at each time point, N = 6 ATP6¹ day 3 and 13, N = 9 ATP6¹ day 6 and 20. Error is S.E.M. Statistical analysis is student's t-test. B) The ATP:ADP ratio analyses show a similar reduction in bioenergetics only in young mutants. N = 9 wildtype at each time point, N = 6 ATP6¹ day 3 and 13, N = 9 ATP6¹ day 6 and 20. Error is S.E.M. Statistical analysis is student's t-test. C) Comparison of uM AMP per mg of tissue between wildtype and ATP6¹ animals show a modest decrease in AMP in aged mutants at day 20. No significant changes are seen in total adenylate pool in ATP6¹ animals. N = 9 wildtype at each time point, N = 6 ATP6¹ day 3 and 13, N = 9 ATP6¹ day 6 and 20. Error is S.E.M. Statistical analysis is student's t-test.