Figure 6. Succinate dehydrogenase and aconitase enzyme activity measurements.

A) Kreb's cycle pathway; intermediates shown in boxes, enzymes shown in red. Gray boxes represent steps where enzyme activity level was measured in isolated mitochondria. B) Aconitase activity measured in wildtype (green) and ATP6¹ (red) mitochondria. ATP6¹ mitochondria have increased aconitase activity compared to wildtype. N = 15 per genotype (0–100 ug mitochondria). Error is S.E.M. Statistical analysis is student's t-test. C) Succinate dehydrogenase activity measured in wildtype (green) and ATP6¹ (red) mitochondria. ATP6¹ mitochondria have reduced succinate dehydrogenase activity compared to wildtype. N = 9 per genotype. Error is S.E.M. Statistical analysis is student's t-test.