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Cells were infected with Ac-SAV3 at 27°C for 1, 2 or 3 days. Next, cells were transferred for 3 days to 12°C, 15°C or 18°C. Whole cell lysates and PEG-precipitated medium fractions were analyzed by WB using α-E2 mabs. Protein fraction quantities, relative to 1 day 27°C followed by 3 days 15°C, are indicated.
