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. 2011 Oct 3;6(10):e25870. doi: 10.1371/journal.pone.0025870

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Kim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Expression of CSN3 and CSN5 mRNA in oocytes during in vitro maturation. For semi-quantitative PCR, single oocyte-equivalent cDNA was used as a template for amplification. GV, GVBD, MI, and MII oocytes were harvested at 0, 2, 8, and 16 h, respectively. Constitutively expressed oocyte-specific H1foo was used as an internal control and synthetic GFP mRNA was added to the lysates before mRNA isolation to compare the efficiency of mRNA recovery. (B) Expression of CSN3 and CSN5 mRNA in follicular components. Oo, oocytes at GV stage; CC, cumulus cells; GC, granulosa cells. Gdf9 and Fshr were used as markers for oocytes and granulosa cells, respectively.