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. 2011 Oct 3;6(10):e25870. doi: 10.1371/journal.pone.0025870

Figure 4. Down-regulation of CSN3 or CSN5 caused meiotic spindle disassembly.

Figure 4

Spindle structure was observed noninvasively using Polscope microscopy. (A) Control MI oocytes cultured for 8 h showed normal barrel-shaped spindles. (B, C) After RNAi treatment followed by 16 h culture, oocytes were arrested at the MI stage and showed no spindle structure. Left panel, bright field; middle panels, dark field; right panels, magnified view of boxed area from the middle panels. Original magnifications ×200 (Left and middle panels). Bar s = 20 µm.