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. 2011 Oct 3;6(10):e25870. doi: 10.1371/journal.pone.0025870

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Kim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Spindles were stained with α-Tubulin antibody (green) and chromosomes were counterstained with propidium iodide (red). (A) Control MI oocytes cultured for 8 h. (B, C) RNAi-mediated knockdown of CSN3 (B) and CSN5 (C) arrested oocytes at the MI stage and the oocytes showed abnormally aggregated spindle and chromosomes. Left panel, spindle structure; middle panel, chromosome; right panel, merged image. Bar = 10 µm.