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MPF and MAPK activities were assessed by measuring the level of phosphorylation of Histone H1, a substrate of MPF, and myelin basic protein, a substrate of MAPK. Oocytes were collected every 2 h during in vitro maturation. Lower panel, relative MPF activity was determined by quantifying phosphorylation of substrates and was expressed relative to that of control oocyte at 2 h.
