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. 2011 Oct 3;6(10):e25870. doi: 10.1371/journal.pone.0025870

Table 1. Sequences of oligonucleotide primers and their expected RT-PCR product sizes.

Gene Accession No. Oligonucleotide Sequencesa AT (°C) Product Size (bp)
CSN3_A b NM_011991 F : CAGCTGCCTAAATACACCTCR : GAGTCATCTTCTTGTGAGCC 60 527
CSN3_B c NM_011991 F : ATCGACCAAGAGATGCTAAAR : CTTTGCAGAGAATGGTTTTC 60 307
CSN5_A b NM_013715 F : CTGTAGAGGGCACAGAAACTR : TCCGACTGCTCTAACTTCTC 60 535
CSN5_B c NM_013715 F : AGGCAACTTGGAAGTGATGGR : TAACATCAATCCCGGAGAGC 60 242
CSN1 NM_145370 F : CAAGGCCGAGTCTACTCCAGR : CTCCAGGAACAGCTTGAAGG 60 295
CSN2 NM_009939 F : CAACAGTGCAGAGATGTGACR : ATAGACGGACACAGTTTTGG 60 459
CSN4 NM_012001 F : TCGGATGCTGGCTACTCTTTR : GGATCTGCTTGTCCCATGTT 60 395
CSN6 NM_012002 F : GAGTGTTTCCGTCGCTCTTCR : CAACCCAGAAACTCCAGCTC 60 261
CSN7 NM_012003 F : TCAGCGGCTAGAGGTTGATTR : TCTGTCAGGTGTTGCTCAGG 60 276
CSN8 NM_133805 F : TCTGTGGAAGAGGATACCACR : TGAAGGTGGATCTTGAACTC 60 497
Gapdh BC092294 F : ACCACAGTCCATGCCATCACR : TCCACCACCCTGTTGCTGTA 60 451
Gdf9 NM_008110 F : GGTTCTATCTGATAGGCGAGGR : GGGGCTGAAGGAGGGAGG 60 446
H1foo NM_138311 F : GCGAAACGGAAAGAGGTCAGAAR : TGGAGGAGGTCTTGGGAAGTAA 60 378
Fshr NM_013523 F : TCCTTCATGGGACTGAGCTTR : AGAGGCTCCCTGCAAAACAT 60 165
a

F, forward; R, reverse.

b

Primers were used for preparation of dsRNA.

c

Primers were used for confirmation the knockdown of target mRNA after RNAi.