Table 1. Sequences of oligonucleotide primers and their expected RT-PCR product sizes.
Gene | Accession No. | Oligonucleotide Sequencesa | AT (°C) | Product Size (bp) |
CSN3_A b | NM_011991 | F : CAGCTGCCTAAATACACCTCR : GAGTCATCTTCTTGTGAGCC | 60 | 527 |
CSN3_B c | NM_011991 | F : ATCGACCAAGAGATGCTAAAR : CTTTGCAGAGAATGGTTTTC | 60 | 307 |
CSN5_A b | NM_013715 | F : CTGTAGAGGGCACAGAAACTR : TCCGACTGCTCTAACTTCTC | 60 | 535 |
CSN5_B c | NM_013715 | F : AGGCAACTTGGAAGTGATGGR : TAACATCAATCCCGGAGAGC | 60 | 242 |
CSN1 | NM_145370 | F : CAAGGCCGAGTCTACTCCAGR : CTCCAGGAACAGCTTGAAGG | 60 | 295 |
CSN2 | NM_009939 | F : CAACAGTGCAGAGATGTGACR : ATAGACGGACACAGTTTTGG | 60 | 459 |
CSN4 | NM_012001 | F : TCGGATGCTGGCTACTCTTTR : GGATCTGCTTGTCCCATGTT | 60 | 395 |
CSN6 | NM_012002 | F : GAGTGTTTCCGTCGCTCTTCR : CAACCCAGAAACTCCAGCTC | 60 | 261 |
CSN7 | NM_012003 | F : TCAGCGGCTAGAGGTTGATTR : TCTGTCAGGTGTTGCTCAGG | 60 | 276 |
CSN8 | NM_133805 | F : TCTGTGGAAGAGGATACCACR : TGAAGGTGGATCTTGAACTC | 60 | 497 |
Gapdh | BC092294 | F : ACCACAGTCCATGCCATCACR : TCCACCACCCTGTTGCTGTA | 60 | 451 |
Gdf9 | NM_008110 | F : GGTTCTATCTGATAGGCGAGGR : GGGGCTGAAGGAGGGAGG | 60 | 446 |
H1foo | NM_138311 | F : GCGAAACGGAAAGAGGTCAGAAR : TGGAGGAGGTCTTGGGAAGTAA | 60 | 378 |
Fshr | NM_013523 | F : TCCTTCATGGGACTGAGCTTR : AGAGGCTCCCTGCAAAACAT | 60 | 165 |
F, forward; R, reverse.
Primers were used for preparation of dsRNA.
Primers were used for confirmation the knockdown of target mRNA after RNAi.