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. 2011 Oct 3;6(10):e25910. doi: 10.1371/journal.pone.0025910

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Marella et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Rats were injected with either PBS, rAAV-GFP, or rAAV-NDI1 in the skeletal muscles. Muscles (tibialis anteriors) were collected at 1 week (1w) or 1 month (1 m) and were stained with Hematoxylin-Eosin (H&E) solutions and infiltrated cells were counted. (A) Representative images of muscle sections from each group after H&E staining. (B) Comparison of the number of infiltrated cells. The histogram shows the number of small mononuclear cells per field of view in the sections separated by at least 90 µm (n = 4 for rats, n = 6 for muscle sections from each animal). Results are expressed as mean ± SD. ** p<0.01 from the respective control (Student's t-test).