Figure 1. Suppression of STAT3 mRNA and protein expression using STAT3 shRNA in pancreatic cancer SW1990 cells.

A, qRT-PCR. Stable STAT3 shRNA and control vector-transfected SW1990 cells and parental SW1990 cells were grown and RNA was isolated for qRT-PCR analysis of STAT3 mRNA expression *P<0.01 compared to SW1990 or control vector-transfected cells. B, Western immunoblot. These cells were grown for total cellular protein extraction and western immunoblot analyses of STAT3 protein expression.