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TEM images of control cells (A, vehicle-only with 5% FBS-DMEM after 24 hr treatment); and cells incubated with 10 μM clofazimine for 24 hr (B) or 87 hr (C). D, E, and F are zoom-in of boxed regions in A, B, and C, respectively. Cytoplasmic objects were categorized into autophagosome-like drug inclusions (aldis) if they did not show characteristic features of the typical organelles, while exhibiting lipid-rich (lamellar or vesicular) internal structures stained with osmium tetroxide.
