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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Cancer Res. 2011 Aug 25;71(19):6270–6281. doi: 10.1158/0008-5472.CAN-11-0838

Fig. 2. Celecoxib induces PKC-mediated GSK3 phosphorylation (B and C) independent of Akt (A).

Fig. 2

A, The indicated cell lines were exposed to the given treatments as indicated for 6 h. LY294002 or wortmannin (Wort.) was pre-incubated with the cells for 30 min before addition of celecoxib (CCB). B, The indicated cell lines were pre-treated with 2 µM Rö31-8220 for 30 min and then co-treated with 50 µM celecoxib for 6 h. C, Calu-1 cells were pre-treated with 20 µM Gö6983, 2 µM GF109203X, 20 µM Rottlerin or 0.5 µM Gö6979 for 30 min and then co-treated with 50 µM celecoxib for 6 h. After the aforementioned treatments, the cells were harvested for preparation of whole-cell protein lysates and subsequent Western blot analysis for the indicated proteins.