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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Neurobiol Dis. 2011 Jul 28;44(3):317–326. doi: 10.1016/j.nbd.2011.07.012

Figure 4. NOX subunit expression in young and aged WT and APP × PS1 mice.

Figure 4

(A) Expression of the regulatory subunit p47phox (p47) was evaluated in tissue homogenates prepared from the frontal cortex of young (4-6 month–old) and aged (16-19 month–old) APP × PS1 and WT mice by Western blot as described in Methods. For consistent quantification across multiple blots, individual samples were normalized first to tubulin and then to levels in WT mice, as described in Methods. Data are mean ± SEM p47expression in 19-20 mice/group, and were analyzed by 2-tailed, unpaired t-tests. (B) Expression of the catalytic subunit NOX2 was evaluated in tissue homogenates prepared from the frontal cortex of young (4-6 month–old) and aged (16-19 month–old) APP × PS1 and WT mice as described in Methods. Data are mean ± SEM NOX2 expression in 19-20 mice/group. (C) Expression of NOX2 homolog NOX4 was evaluated in tissue homogenates prepared from the frontal cortex of young (4-6 month–old) and aged (16-19 month–old) APP × PS1 and WT mice as described in Methods. Data are mean ± SEM NOX4 expression in 19-20 mice/group, and were analyzed by 2-tailed, unpaired t-tests. ** indicates significant (p < 0.01) increases in NOX4 expression in aged APP × PS1 mice as compared to aged WT mice. (D) Representative images depict the pattern of NOX4 expression in young and aged APP × PS1 and WT mice.