Table 1.

Analysis of composition and morphologies of amelogenin assemblies during Mmp-20 proteolysis. Mass percentage of protein bands corresponding to the cleavage products, stained in SDS-PAGE and estimated by ImageJ software. The dotted line (----) indicates products that were not detected. The main morphology of the assemblies corresponding to specific proteolysis time was accessed by TEM and particle size distribution was estimated by ImageJ software.

TIME	0.1 hr	1 hr	2 hrs	4 hrs	10 hrs	20 hrs
rP172(*)	95.38	61.11	45.98	24.62	5.66	1.28
2–162	----	14.45	17.24	21.78	22.64	24.3
2-148	4.62	24.44	36.78	49.24	56.60	53.71
13k	----	----	----	----	5.66	6.39
11k	----	----	----	----	2.83	3.32
Extended TRAP (1-63)	----	----	----	1.52	1.89	3.84
TRAP (1-45)	----	----	----	2.84	4.72	7.16
rP172: 2-148/2-162	~20:1	~2:1	~1:1	~1:3	~1:15	~1:80
(#) Diameters (nm)	14.36	13.56	11.53	11.83	11.16	10.69
Main Morphology	Spherical particles with some pearl-like chains	Spherical particles with longer pearl-like chains	Nanorods with their evolution patterns	Nanorod evolution patterns	Nanorod evolution patterns, some aggregates	Large globular aggregates

^(*)As control measurements, the average diameters were 19.27 nm and 15.16 nm respectively for pure rp172 and pure rp148 incubation within the pH8.0 Tris-Cl buffer at 37 °C, larger aggregation diameters of rp148 are not accounted.

^(#)Particle size, as for the chain-like microstructures, the size represents the average diameters of the chains, N=50.