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. Author manuscript; available in PMC: 2012 Oct 3.
Published in final edited form as: Mol Pharm. 2011 Aug 17;8(5):1941–1954. doi: 10.1021/mp200309x

Figure 5.

Figure 5

Gel mobility shift assay analysis of the interaction of a 22 bp duplex containing the 1,2-GG intrastrand cross-link of cisplatin (22-TGGT) or adducts of 1 or 2 (22-TCGT) with HMGB1a and HMGB1b. Radioactively labeled oligodeoxyribonucleotide duplexes (1 pmol) were incubated with 10 ng of HMGB1a or 40 ng of HMGB1b. A. Sequences of the synthetic oligodeoxyribonucleotides used in this study. B, C. Autoradiograms of the gel mobility shift assay analysis of the interaction with HMGB1a (B) and HMGB1b (C). Lanes: 1,2 - control (unplatinated) duplex; 3,4 – 1,2-GG intrastrand cross-link of cisplatin (22-TGGT); 5,6 – 22-TCGT duplex modified by 1; 7,8 – 22-TCGT duplex modified by 2. Lanes 1,3,5,7 – no protein; lanes 2,4,6, 8 – 10 ng of HMGB1a (B) or 40 ng of HMGB1b (C).