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. 2011 Sep 5;4:169. doi: 10.1186/1756-3305-4-169

Figure 2.

Figure 2

Relative quantification using RT-qPCR of mRNA expression of 12 genes in five resistant phenotypes (TcR1x-TcR5x) compared with the sensitive strain. The mRNA levels were normalized using the expression of the reference gene (HGPRT). The relative quantification results were obtained using the REST2009 program by randomization test with 10,000 permutations (p < 0.05). The graph shows the data of each gene evaluated for each of the resistant phenotypes (white bars correspond to TcR1x-TcR4x; black bars represent TcR5x phenotype) in triplicate and two independent experiments.