Table 1.
Sequences of primers used to amplified T.cruzi genes using qPCR
| Function | Genes/Genebank ID | Size | Forward | Reverse |
|---|---|---|---|---|
| Activation system | Old Yellow enzyme (OYE)1/AB075599 | 146 bp | 5'-ACTTTCGCTTGCCTATCTGC-3' | 5'-GTATTTGCTGGTCTGCCTCTTC-3' |
| Nitroreductase I (NTR)1/XM805552 | 195 bp | 5'-GCACGTGATTGGTATGGATG-3' | 5'-CTTTGTTGGGTCAAATCGCT-3' | |
| NADPH-cytochrome P450 reductase (P450)1/DQ857724 | 140 bp | 5'-GCATACCGGTTGGACACTTT-3' | 5'-GCCTTCAGGAATGATACGGA-3' | |
| Detoxification system of free radicals | Trypanothione reductase (TRYT)1/M38051 | 191 bp | 5'-CTCTACAAGAAGCGGGTTGC-3' | 5'-CTGAGAGTGGTGCGATCAAA -3' |
| Glutathionyl spermidine synthetase (GTS)1/XM_815753.1 | 125 bp | 5'-ACTTCCACCGGGTCTTTTCT-3' | 5'-CTGCGGCATTCATCACATAC -3' | |
| Trypanothione synthetase (TS)1/XM_805507.1 | 130 bp | 5'-ATCCGTTGGAGGATGAAGTG-3' | 5'-TAAATGTCAGACGACGCAGC-3' | |
| Cytosolic tryparedoxin peroxidase (cTXP)2/AJ012101 | 169 bp | 5'-AAGTGGCTGGTGCTCTTCTT-3' | 5'-TTGCGCTCAATGCTTGTCCA-3' | |
| Mitochondrial tryparedoxin peroxidase (mTXP)2/AJ006226.1 | 137 bp | 5'-TGCAACACCCTGCGACTTCTTA-3' | 5'-GCCCTTGTAGTCATTCAAGCTG-3' | |
| Superoxide dismutase-A (SOD)1/U90722 | 184 bp | 5'-GTTGAGACGTGCGGTGAATA-3' | 5'-GCCTTGTGGTGTTTGGTGTA-3' | |
| Extracellular flow of drugs system | Multi-drug resistant gene (MDR)1/XM_806226.1 | 188 bp | 5'-ATCGCTTCTATGACCCTTCCTC-3' | 5'-GGCTTCCTCCACTTCTTCGT-3' |
| Folate metabolism system | pteridine reductase-1 (ptr1)1/AF174398 | 157 bp | 5'-ACAGTATCGCTGTGCGTCTG-3' | 5'-AGAGAGCTGCCGAGACTGAG-3' |
| Methionine adenosyltransferase (MAT)1/XM_799937 | 140 bp | 5'-CTACGCCGTAATGGGACACT-3' | 5'-TGTTGGGCCGAGATAAGAAC-3' | |
| Dihydrofolate reductase-thymidylate synthase (DHFR-TS)1/XM_810234 | 164 bp | 5'-GTGGCGGTAAATGGTGGACT-3' | 5'-ATGGTGGTGCGGTAAATAGC-3' | |
| Spermidine putrescine transporter (EPT)2/XM_805310.1 | 152 bp | 5'-TGAATCCACCGTTGCGGGTCT-3' | 5'-CGCATTCTTGACGCTCTGCCT-3' | |
| Celullar metabolism system | Histone H1 (H1)2/L27119.1 | 124 bp | 5'-GCAGCAGCCAAGAAGGCTGT-3' | 5'-CGACAAAGCACGGTTGAGCCA-3' |
| Zinc finger protein (ZN)2/XM_808517 | 132 bp | 5'-TGCAGCACGTTCCATTGTGCC-3' | 5'-CGCTGCCGCCCGTAGTTTAAT -3' | |
| L-threonine dehydrogenase (TDH)2/XM_807811 | 166 bp | 5'-TGAACCCCTCMACGGTKTACGGTGT-3' | 5'-GTACATRTGAATGGCGTAGTCYGTGG-3' | |
| Glycoprotein gp82 (gp82)1/L14824 | 118 bp | 5'-GCCAACGATAAAGGCAGTGT-3' | 5'-GTGTGGAAGAAGCGGAGAAG-3' | |
| Cruzipain (CZP)2/X54414 | 135 bp | 5'-CATCGGTGAGGTCCTCTGTT-3' | 5'-CTGTGGTATGGCTGATAGCG-3' |
1: Genes analyzed for all resistant phenotypes (TcR1x-TcR5x) and the parental sensitive strain
2: Genes analyzed only for the parental sensitive strain and the TcR5x resistant phenotype.