Table 3.
Effects of drug interactions between components of different combinations
Modalities | Cell Line | Combination Index (CI1) | Drug Alone (×IC50) |
Dose Reduction Index (×IC50) | R3 | Effect4 | ||||
---|---|---|---|---|---|---|---|---|---|---|
IC2 50 | PNP | Doc | Car | PNP | Doc | Car | ||||
Docetaxel Carboplatin |
SKOV5 | 0.96 | - | 5.01 | 1.34 | - | 2.7 | 1.68 | 0.98 | Additive + |
OVCAR6 | 0.89 | - | 2.76 | 2.16 | - | 2.1 | 2.44 | 0.99 | Additive + |
|
PNP-GDEPT Docetaxel |
SKOV | 0.43 | 8.22 | 5.01 | - | 6.12 | 3.7 | - | 0.98 | Synergy +++ |
OVCAR | 0.21 | 2.03 | 2.76 | - | 7.97 | 10.82 | - | 0.93 | Strong Synergy ++++ |
|
PNP-GDEPT Carboplatin |
SKOV | 0.29 | 8.22 | - | 1.34 | 12.36 | - | 4.7 | 0.97 | Strong Synergy ++++ |
OVCAR | 0.28 | 2.03 | - | 2.16 | 5.66 | - | 9.08 | 0.96 | Strong Synergy ++++ |
|
GDEPT Docetaxel Carboplatin |
SKOV | 0.37 | 8.22 | 5.01 | 1.34 | 13.96 | 8.51 | 5.3 | 0.96 | Strong Synergy ++++ |
OVCAR | 0.11 | 2.03 | 2.76 | 2.16 | 19.89 | 27.02 | 31.9 | 0.94 | Very Strong Synergy +++++ |
1 Combination index values generated when drugs were combined at constant ratio of 1:1 e.g. IC25 of docetaxel with IC25 of carboplatin; 2ED, Effective dose, which can result in 50, 75 and 90% of cell killing; 3The linear correlation coefficient, R, of the median-effect plot. The acceptable range of 'R' values varies with the type of system used; Enzyme or receptor systems (r > 0.96), tissue culture systems (r > 0.90) and animal experiments (r > 0.85); 4Combination effects, CI value 0.90-1.10 signifies additivity (+); CI 0.7-0.85 moderate synergism (++); CI 0.85-0.9 slight synergies (+++); CI 0.30-0.70 synergism (++++); CI 0.10-0.30 strong synergism (+++++) and CI 0.01-010 very strong synergism (++++++); 5 represents SKOV-3; 6 represents OVCAR-3