Expression of Pdf2.1, Pdf2.2, and Pdf2.3 promoter::GUS fusions in syncytia induced by H. schachtii. GUS expression driven by the Pdf2.1 promoter in nematode feeding sites at 5 dpi and 15 dpi (A, B). Root tissues surrounding the syncytium did not show any GUS expression. For Pdf2.2, there was also GUS expression in syncytia at 5 dpi and 15 dpi (C, D). However, this expression was not limited to the syncytium but was also found in surrounding tissues. For Pdf2.3, there was also GUS expression in syncytia as well as in surrounding tissues at 5 dpi and 15 dpi (E, F). This expression, especially at 5 dpi, was more intense in surrounding tissues than in syncytia. N, nematode; S, syncytium