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. 2011 Oct;49(10):1100–1107. doi: 10.1016/j.plaphy.2011.07.005

Fig. 6.

Fig. 6

In situ RT-PCR. In situ RT-PCR analysis of Pdf2.1, Pdf2.2, and Pdf2.3 on sections of syncytia induced by Heterodera schachtii in roots of Arabidopsis as well as uninfected control roots. A, B and C – transcripts of Pdf2.1, Pdf2.2, and Pdf2.3, respectively, accumulate in the 5 dpi syncytia. D, E and F – control sections of 5 dpi syncytia show lack of the specific signal of Pdf2.1, Pdf2.2, and Pdf2.3, respectively. G, H and I – specific staining of Pdf2.1, Pdf2.2, and Pdf2.3, respectively, in 15 dpi syncytia. J, K and L – control sections of 15 dpi syncytia show lack of the specific staining of Pdf2.1, Pdf2.2, and Pdf2.3, respectively. M – very weak staining of Pdf2.1 within the central cylinder of the uninfected root. N – transcripts of Pdf2.2 accumulate in cells of the central cylinder of the uninfected root. O – transcripts of Pdf2.3 accumulate in cells of the central cylinder of the uninfected root. P, R and S – control sections of uninfected control roots show lack of the specific signal of Pdf2.1, Pdf2.2 and Pdf2.3, respectively. A–F and M–S scale bar = 20 μm; G–L scale bar = 50 μm.