Table 3.

Total intracellular GSH levels in CGNs cultured alone or with cerebellar astrocytes

Astrocytes	CGNs	Control	+DE-71
None	Gclm (+/+)	13.5 ± 1.0	11.7 ± 1.5
Gclm (+/+)	Gclm (+/+)	17.9 ± 1.1 *	18.6 ± 1.9*
Gclm (−/−)	Gclm (+/+)	15.2 ± 1.4	14.6 ± 1.4
None	Gclm (−/−)	2.6 ± 0.7	3.1 ± 0.9
Gclm (+/+)	Gclm (−/−)	8.3 ± 1.1**	6.7 ± 0.8*
Gclm (−/−)	Gclm (−/−)	3.8 ± 0.8	3.5 ± 0.7

Astrocytes and neurons were co-cultured as described in Methods and treated for 24 hr with DE-71 (10 μM). Total intracellular GSH levels were measured as described by Giordano et al. (2006). Values are expressed as nmol/mg protein, and results represent the mean (± SD) of three separate experiments.

^*Significantly different from CGNs alone (without astrocytes), p<0.01;

^**p<0.001.