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. 2011 Aug 12;12(10):1018–1023. doi: 10.1038/embor.2011.152

Figure 1.

Figure 1

The zinc-finger domain of Krox20 mediates interaction with Ubc9. (A) Growth of yeast transformed with the indicated constructs was tested on non-selective and selective media. Bait and prey constructs were based on Gal4 DNA-binding domain (G4DB) and Gal4 activation domain (G4AD) vectors, respectively. (B) Pull-down experiments were carried out with immobilized purified glutathione S-transferase (GST) or a GST–Ubc9 fusion and in vitro translated, radioactively labelled Krox20. (C) Deletion constructs of Krox20 were tested for interaction with Ubc9 by yeast two-hybrid screening as indicated in A. TA, transactivation domain; ZF, zinc finger.