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. 2011 Sep 9;12(10):1055–1061. doi: 10.1038/embor.2011.175

Figure 4.

Figure 4

LGR4 and LGR5 are required for Wnt/PCP signalling in Xenopus. (A,B) Rspo3 signalling requires LGR4 to induce gastrulation defects. Embryos were injected equatorially at 4-cell stage with morpholinos and/or messenger RNA (mRNA) as indicated (250 pg xRspo3 mRNA, 10 ng (+) and 20 ng (++) of LGR4 Mo1). Scale bar indicates 500 μm. (C–E) ATF2-luciferase reporter activity is reduced by LGR4/5 Mos in Xenopus embryos. Embryos were injected equatorially with ATF2-luc reporter (100 pg) and Renilla reporter plasmids (25 pg) and the indicated morpholinos (20 ng LGR4 Mo1, 5 ng LGR5 Mo1 in E) and mRNAs. Luciferase reporter assays were performed from whole embryos collected at gastrula stage. Luciferase activity in embryos injected with either Co Mo or Co Mo plus mRNA of the indicated activators within each condition were set to 100%. RLA, relative luciferase activity. All experiments were performed at least twice with three replicates each. Co, control medium; Mo, morpholino oligonucleotide.