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. 2011 Jul 4;39(18):8258–8270. doi: 10.1093/nar/gkr314

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Temperature dependent expression of the agsA-(4U-hp2)-bgaB fusion in DH5α E. coli cells. Following RNA constructs were investigated: 4U-hp2-wt (filled diamonds), 4U-hp2-A8C-mutant (open triangles), 4U-hp2-G14A-C25U (filled triangles). Expression levels were referenced to the respective expression level of the 4U-hp2-wt at 30°C which was set to 1.