Table 3.
Thermodynamic parameters of Mg2+ binding as well as macroscopic and microscopic dissociation constants of the two-binding-site-model (Figure 1)
| Kdiss (mM) | |
|---|---|
| K1 | 3.58 ± 0.67 mM |
| K2 | 2.06 ± 0.66 mM |
| K2,4 | 1.17 ± 0.12 mM |
| K3,4 | 0.85 ± 0.12 mM |
| K1,2 | 6.31 ± 2.43 mM |
| K1,3 | 8.68 ± 3.71 mM |
| ΔGK1 (10°C) | −13.262 ± 0.446 kJ/mol |
| ΔGK2 (10°C) | −14.556 ± 0.780 kJ/mol |
| ΔGges (10°C) | −27.818 ± 0.899 kJ/mol |
| ΔGCD (50.24°C) | −18.860 ± 0.143 kJ/mol |
| ΔGges (37°C) | −21.807 ± 0.443 kJ/mol |
| ΔHges | −90.855 ± 6.469 kJ/mol |
| T*ΔSges (10°C) | −63.037 ± 6.404 kJ/mol |
ΔGK1, ΔGK2 and ΔGges were calculated for a temperature of 10°C. ΔGCD (50.24°C) was derived from CD melting studies. ΔHges, T*ΔSges and ΔGges (37°C) were calculated from the difference of ΔGges (10°C) and ΔGCD (50.24°C). Errors in K1, K2, K2,4, K3,4 and KCD (Table 2) are statistical errors resulting from the fit. Errors in K1,2, K1,3, ΔGK1 (10°C), ΔGK2 (10°C), ΔGges (10°C), ΔGCD (50.24°C), ΔHges and T*ΔSges (10°C) are Gaussian errors calculated by error propagation.