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. 2011 Jun 30;39(18):8105–8121. doi: 10.1093/nar/gkr508

Figure 1.

Figure 1.

(A) Diagram of the steps required for the assembly of the primary subunits of the 90S pre-ribosome onto the 35 pre-rRNA. The initial binding of the tUTP subunit, which occurs at an early and independent step (step 1), is required for the recruitment of other components and subunits through two independent assembly branches. One of these branches requires the UTP-B and U3 snoRNP subcomplexes (step 2). The other branch involves the stepwise binding of Rrp5 and the UTP-C subunit (step 3). The assembly of other factors through each one of these two branches might be concurrent or subsequent to the association of the primary building subunits (question marks). Nan1, Pwp2, Rrp5 and Rrp7 are the proteins of the 90S particle that have been epitope tagged or conditionally depleted in different experiments of this study. (B) Schematic outline of the experimental strategy used in this work. The aim of this study was to analyze the assembly behavior of specific 90S pre-ribosome components in the presence and absence of other 90S pre-ribosome proteins. Details about the purpose of the techniques and steps outlined in this Figure are given in the text at the beginning of the ‘Results’ section.