Figure 4.

Protein composition analysis of the complexes formed by Utp20, Imp4 and Bms1 in wild type and Rrp5-, Nan1- or Pwp2-depleted cells. The MYC-tagged proteins used as baits and the proteins depleted before complex purifications are indicated on the top. The proteins co-purifying with the baits that were identified in these analysis, the designations of the corresponding open reading frames, and the subcomplex they belong to are indicated in the three first columns and in the last column. The five proteins highlighted in red are not components of the known primary UTP subunits, but consistently co-purify with Pwp2 in our purifications from both wild-type and Rrp5-depleted cells. A shaded rectangle indicates that the protein was found associated to the bait.