Figure 7.

Protein composition analysis of pre-ribosomal complexes in cells depleted of Nan1, Rrp5, Utp20, Imp4 or Bms1. The MYC-tagged proteins used as baits, and the proteins depleted before complex purifications are indicated on the top. The proteins co-purifying with the baits that were identified in these analyses, the designations of the corresponding open reading frames and the subcomplex they belong to are indicated in the three first columns and in the last column. A subset of 16 components of the 90S pre-ribosome that consistently co-purify with Pwp2 in our analysis, both in wild-type and Rrp5-depleted cells, are dot-marked in the second column on the left. A shaded rectangle indicates that the protein was found associated to the bait.